Understanding stenosis-induced platelet aggregation on a chip by high-speed optical imaging

Vascular stenosis is a pathological hallmark of atherosclerosis, the leading cause cardiovascular diseases such as stroke and myocardial infarction. While stenosis-induced thrombus formation has been extensively studied using in vivo vitro vascular models, transient dynamic process platelet aggregation remains unclear due to lack analytical tools with both high spatial temporal resolution. Here we report spatiotemporally resolved observation shear-induced an microfluidic model optical time-stretch imaging system. Specifically, characterized size, shape, population aggregates at single-cell resolution every 20 min presence different agonists 3D model. Our results indicate significant enhancement when agonist were present, thus suggesting synergistic effect atherogenic blood flow disturbance circulating factors on activation. In particular, platelets activated by thrombin receptor activator peptide 6 (TRAP-6) led broad-sized distribution significantly large (>240 µm 2 area). These findings are expected deepen our understanding mechanism behind pave way for development better antithrombotic therapeutics. • A chip used platform study stenosis. microscope employed aggregation. Imaging show TRAP-6 causes preferential